How to Find Starch, Protien and glucouse in Unkown Substance essays

How to Find Starch, Protien and glucouse in Unkown Substance essays Day 1: What organic or inorganic compounds (starch, protein, glucose, NACL) are in an unknown solution. Day 2: Which of the substances (starch or NACL) will be able to diffuse out of the plastic bag or cell if you will. Hypothesis: If the starch and NACL compounds do diffuse through the plastic bag, then we know that these substances will diffuse in an actual cell also. Dialysis tubing, thread, 100 mL beaker, 10 mL graduated cylinder, unknown mixture, food test reagents, test tubes, hot water bath. Step 1- Put on safety goggles and apron. Step 2- First, set up four test tubes on the lab surface; held in place upright. Then pour 2 mL of the unknown solution into the first test tube. Then use the bottle of Biruet reagent to drop exactly five drops directly into the first test tube. Observe the color of the mixed solution. If it turned Indigo then we can conclude that the unknown solution does in fact contain protein. Step 3- Second, pour exactly 2 mL of the unknown solution into the second test tube and then use the bottle of Iodine to drop exactly 3 mL directly into test tube two. If, after carefully observing the change in color; it turned a dark blue then we can conclude that the unknown solution does indeed contain starch. Step 4- Third, pour exactly 5 mL of the unknown solution into the third test tube. Using the Benedicts reagent drop exactly 3 mL directly into the third test tube. Then using the spatula take hold of the test tube and bring it to the heater where you will find a glass container filled with boiling water. Carefully place the test tube in the water and let it sit for exactly five minutes. Finally, Take the test tube out of the boiling water and observe the change in color of the solution. If the color turned a Burnt orange then we can conclude that the unknown solution does in fact contain glucose. ...